Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific MES, Anhydrous, 25 G
MES, Anhydrous, 25 G, is a small-volume, high-purity buffering agent used to maintain pH stability in laboratory experiments. With its anhydrous form, it dissolves readily in solvents for precise pH adjustments. MES buffers in the pH range of 5.5 to 6.7, making it particularly useful in applications such as electrophoresis, enzyme assays, and in preparing cell culture media. The 25 g package is ideal for small-scale experiments or for laboratories that require frequent pH adjustments but do not need larger quantities of the reagent. MES is an essential tool for many biochemical and biological processes that require exacting conditions for reproducible results.
AG Scientific Inc MOPS Free Acid 1 KG
MOPS, also known as 3-(N-morpholino)propanesulfonic acid, is one of Good’s buffers. It is N-substituted amino sulfonic acid with a morpholinic ring and has zwitterionic and inert properties. MOPS buffer is broadly used in biological and biochemical studies. It can be mixed with MOPS sodium salt to attain desired pH.MOPS is recommended as an agent for non-coordinating buffers in solutions with metal ions because it hardly forms a complex with most metal ions such as Cu, Ni, Mn, Zn and Co ions. The Good's buffer interferes with the Folin protein assay and partially decomposes when autoclaved in the presence of glucose. MOPS can be used as a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 49 Grams of Powder, Makes 10 Liters of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (10 liters, 49 grams of powder) is designed for plant tissue culture applications that require large volumes of prepared solution. This medium is enriched with essential nutrients, growth regulators, and MES buffer, which helps to stabilize the pH during plant tissue growth. The formula ensures optimal growth conditions for plant cells, tissues, and organs in vitro, making it ideal for shoot multiplication, regeneration, and propagation experiments. It is commonly used in commercial plant propagation, agricultural research, and plant breeding. The large 10-liter yield makes this medium suitable for labs and research facilities with higher throughput needs.
eMolecules 58884-35-8 | 4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol | Broadpharm | MFCD24716961 | 188.190 | C9H8N4O | 98.000 | Cc1nnc(nn1)-c1ccc(O)cc1 | 1g | 349512533
4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol | Broadpharm | 58884-35-8 | MFCD24716961 | 188.190 | C9H8N4O | 98.000 | Cc1nnc(nn1)-c1ccc(O)cc1 | 1g | 349512533
Research Products International Corp HEPES, Sodium Salt, 5 KG
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659807.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G
HEPES, Free Acid is a biochemical reagent used for maintaining a stable pH environment in biological and chemical applications. This 250g package is ideal for preparing buffered solutions for use in cell culture, protein analysis, and other biochemical experiments. HEPES, or 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, is widely used because of its excellent buffering capacity in the pH range of 6.8 to 8.2. It is commonly used in cell biology to maintain physiological pH during experiments. This reagent is highly stable, non-toxic to cells, and is ideal for applications that require precise pH control over extended periods of time.
